General Western Blot Reagents
Bioworld Nylon Membrane Stripping Buffer (10X), 250 mL
Product DescriptionNylon Membrane Stripping Buffer (10X) is a liquid used for the hybridization of probes to nucleic acids. Buffer contains Tris HCL at pH 8.0 (1mM), EDTA (100mM), and Denhardt’s solution (1X). To use this product, dilute at 10-fold with sterile deionized water and strip the membrane by 2 hours of incubation at 75°C.
Bioworld Nitrocellulose Stripping Buffer (10X), 250 mL
Product DescriptionNitrocellulose stripping buffer (10X) is used for hybridization of probes to nucleic acids. It is ideal for removal of secondary antibody after detection of highly abundant targets. It is also used for nucleic acid and protein related blotting.This buffer contains 0.5X SSC and 100 mM EDTA at pH 8.0. To strip the membrane, dilute buffer to 1X in water. Warm solution to 50-70°C and perform 2 to 3 changes (15 minutes each) of boiling stripping buffer.
Bioworld Nitrocellulose Stripping Buffer (10X), 500 mL
Product DescriptionNitrocellulose stripping buffer (10X) is used for hybridization of probes to nucleic acids. It is ideal for removal of secondary antibody after detection of highly abundant targets. It is also used for nucleic acid and protein related blotting.This buffer contains 0.5X SSC and 100 mM EDTA at pH 8.0. To strip the membrane, dilute buffer to 1X in water. Warm solution to 50-70°C and perform 2 to 3 changes (15 minutes each) of boiling stripping buffer.
Bioworld Blot Stripping Buffer II (4X), 500 mL
Product DescriptionBlot stripping buffer II (4x) contains enough liquid concentrate to make 4L of 1X solution. Product is 0.2M glycine and has a pH value of 2.5.To use this product, place the membrane into stripping buffer for 30-45 minutes with gentle agitation. Buffer should be heated to approximately 50°C for incubation. Wash membrane 3 times with TBST or PBST before reprobing.Product is ultra pure grade.
Bioworld Re-Probe Solution western Blots, 125 mL
Re-Probe Solution, Western BlotsRe-Probe is supplied as 5X. The 125ml kit sufficient for 25 blots. Not recommended for chromogenic substrates.
Bioworld Nylon Membrane Stripping Buffer (10X), 500 mL
Product DescriptionNylon Membrane Stripping Buffer (10X) is a liquid used for the hybridization of probes to nucleic acids. Buffer contains Tris HCL at pH 8.0 (1mM), EDTA (100mM), and Denhardt’s solution (1X). To use this product, dilute at 10-fold with sterile deionized water and strip the membrane by 2 hours of incubation at 75°C.
ProteinSimple Anti-Mouse Detection Module, 1ea
Use with a Wes or Peggy Sue/Sally Sue a Separation Module. Includes: Luminol-S, Peroxide, Antibody Diluent 2, Anti-Mouse Secondary Antibody, Streptavidin-HRP.
ProteinSimple Anti-Rabbit Detection Module, 1ea
Use with a Wes or Peggy Sue/Sally Sue Separation Module. Includes: Luminol-S, Peroxide, Antibody Diluent 2, Anti-Rabbit Secondary Antibody, Streptavidin-HRP.
ProteinSimple RePlex Module for Jess
This RePlex Module provides all the reagents needed to remove primary and secondary antibodies in a RePlex assay with two immunoassays or immunoassay with Total Protein assay in a single run. Please note this module can only be used with Jess.
ProteinSimple Total Protein Detection Module for Jess, Wes, Peggy Sue or Sally Sue
Use with a Jess/Wes or Sally Sue/Peggy Sue Separation Module. Includes: Luminol-S, Peroxide, Antibody Diluent 2, Total Protein Reconstitution Agent 2, Total Protein Streptavidin-HRP.
ProteinSimple No secondary Detection Module
Compatible with Jess, Abby, Wes, Peggy Sue and Sally Sue systems. Module includes: Luminol-S, Peroxide, Antibody Diluent 2, Streptavidin-HRP.
ProteinSimple EZ Standard Pack 1, (8) Single Use Biotinylated Ladder and Single Use FL Standard
8 pk, EZ Standard Pack 1
LabScientific NONFAT DRY MILK standard for blotting. Molecular Biology Grade. Western blot. Biotin-Free. 2KG
Standard for all blotting: Western Blot Grade - Tested - Molecular Biology Grade Biotin-Free. 2KG